Pengaruh Hormon 2.4-D (2,4-Dichlorophenoxyacetic Acid) Terhadap Kultur Kalus Daun Tembakau (Nicotiana tabacum L)

Authors

  • Welsiliana Welsiliana Program Studi Biologi Universitas Timor
  • Meri Helsiana Mata Program Studi Biologi Universitas Timor

DOI:

https://doi.org/10.32938/jsb/vol5i1pp39-44

Keywords:

hormon 2.4-D, kalus, tembakau

Abstract

Budidaya tanaman melalui teknik kultur jaringan selalu mengalami perkembangan dalam menghasilkan bibit unggul. Keberhasilan kultur jaringan sangat dipengaruhi oleh faktor lingkungan yang meliputi media dan penggunaan zat pengatur tumbuh. Penelitian ini bertujuan untuk melihat pengaruh zat pengatur tumbuh (hormon) dalam menginisiasi kalus pada tanaman tembakau. Metode penelitian diawali dengan pembuatan larutan stok dan pembuatan media Murashige dan Skoog (MS) serta dilanjutkan dengan kultur eksplan daun tembakau. Hasil penelitian menunjukkan bahwa pemberian hormon 2.4-D (2 ppm) pada eksplan daun tembakau dapat merangsang terbentuknya kalus hingga menghasilkan diameter 0.6 cm di minggu ke-4.

Author Biographies

Welsiliana Welsiliana, Program Studi Biologi Universitas Timor

Program Studi Biologi Universitas Timor

Meri Helsiana Mata, Program Studi Biologi Universitas Timor

Program Studi Biologi Universitas Timor

References

Edwards, K.D., Fernandez-Pozo, N., Drake- Stowe, K., Humphry, M., Evans, A. D., Bombarely, A., Allen, F., Hurst, R., White, B., Kernodle, S.P., Bromley, J.R., Sanchez-Tamburrino, J.P., Lewis, R.S., & Mueller, L.A. 2017.A reference genome for Nicotiana tabacum enables map-based cloning of homeologous loci implicated in nitrogen utilization efficiency. BMC Genomics, 18: 448. https://doi.org/10.1186/s12864-017- 3791-6

Erawati, D. N., Taufika, R., & Adiwinata, D.A. 2023. Pengaruh Pemberian Sitokinin Terhadap Kalus Tembakau Varietas Na-Oogst (Nicotiana tabacum L.) Melalui Kultur In Vitro. AGROPROSS: National Conference Proceedings of Agriculture, 288-295. https://doi.org/10.25047/agropross.2 023.40

Desriatin, N. L. 2010. Pengaruh Kombinasi Zat Pengatur Tumbuh IAA dan Kinetin terhadap Morfogenesis pada Kultur In Vitro Tanaman Tembakau (Nicotiana tabacum L. var. Prancak- 95). Kultur Jaringan Tembakau. Surabaya: Institut Teknologi Sepuluh November.

Hesami, M., & Daneshvar, M. H. 2018. In vitro adventitious shoot regeneration through direct and indirect organogenesis from seedling- derived hypocotyl segments of Ficus religiosa L.: an important medicinal plant. HortScience, 53(1): 55-61. https://doi.org/10.21273/HORTSCI12 637-17

Lang, J., Pankowski, J., Grabarz, P., Pluciński, B., & Jedynak, P. 2020. Comparing the effects of different exogenous hormone combinations on seed- derived callus induction in Nicotiana tabacum. Biochimica Polonica, 67(4): 449-452. https://doi.org/10.18388/abp.2020_5 456

Lestari, E. G. 2011. Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal Agro Biogen, 7(1): 63-68. https://doi.org/10.21082/JBIO.V7N1. 2011.P63-68

Lestari, E., Nurhidayati, T., & dan Nurfadilah, S. 2013. Pengaruh Konsentrasi ZPT 2,4-D dan BAP terhadap Pertumbuhan dan Perkembangan Biji Dendrobium laxiflorum J.J Smith secara In Vitro. Jurnal Sains Dan Seni Pomits, 2 (1): 43-47. https://doi.org/10.12962/J23373520. V2I1.2748

Mahadi, I., Syafi’i, W., & Sari, Y. 2016. Induksi Kalus Jeruk Kasturi (Citrus microcarpa) Menggunakan Hormon. Jurnal Ilmu Pertanian Indonesia (JIPI), (21)2: 84-89. https://doi.org/10.18343/jipi.21.2.84

Mata, M. H. 2021. Akumulasi α-Tokoferol pada Organ Tanaman dan Kultur Suspensi Sel Jatropha gossypiifolia Linn dari Nusa Tenggara Timur. Jurnal Saintek Lahan Kering, 4(1): 12-15. https://doi.org/10.32938/slk.v4i1.141 1

Meneses, A., Flores, D., Munoz, M., Arrieta, G., & Espinosa, A. M. 2005. Effect of 2.4-D, Hydric stress and light on indica rice (Oryza sativa) somatic embryogenesis. Rev Biol Trop (Int J). 53(3-4): 361-368. https://doi.org/10.15517/RBT.V53I3- 4.14598

Muharyati, Y., Defiani, M.R., & Astiti, N.P.A. 2015. Pertumbuhan Anggrek Vanda helvola Pada Media Yang Diperkaya Jus Tomat. Jurnal Metamorfosa, 2(2): 66-71. https://doi.org/10.24843/metamorfo sa.2015.v02.i02.p03

Murashige, T., & Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Plant Physiol, 15:473-497.

Purnamaningsih, R. 2006. Induksi Kalus dan Optimasi Regenerasi Empat Varietas Padi melalui Kultur In Vitro. Jurnal AgroBiogen. 2(2):74-80. https://doi.org/10.21082/jbio.v2n2.2 006.p74-80

Purwaningrum, Y. 2013. Kultur Kalus Sebagai Penghasil Metabolit Sekunder Berupa Pigmen. AGRILAND, 2 (2): 117-127.

Putri, A.B.S., Hajrah., Armita, D., & Tambunan, I.R. 2021. Teknik kultur jaringan untuk perbanyakan dan konservasi tanaman kentang (Solanum tuberosum L.) secara in vitro. Filogeni: Jurnal Mahasiswa Biologi, 1(2): 69-76. https://doi.org/10.24252/filogeni.v1i 2.23801

Restanto, D.P., Wiranegara, A., Dewanti, P., Kristanto, B., & Avivi, S. 2021. Pengaruh hormon 2,4- dichlorophenoxyacetic acid Terhadap Induksi Kalus Tanaman Sorgum (Sorghum bicolor (L.). Agritrop: Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 19(1): 12-18. https://doi.org/10.32528/AGRITROP. V19I1.5463

Shinde, S.S., Sanap, S. B., Patil, A. M., Shinde, N. A., Daspute, A. A., & Sarode, D. K. 2021. In vitro regeneration of Nicotiana tabacum L. from callus culture. The Pharma Innovation Journal, 10(12): 904-908. https://doi.org/10.13140/RG.2.2.221 31.30248

Sitorus, E. N., Hastuti, E. D., & Setiari, N. 2011. Induksi Kalus Binahong (Basella rubra L.) Secara In Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda. Bioma. 13 (1): 1410-8801. https://doi.org/10.14710/bioma.13.1 .1-7

Wati, T., Astarini. I. A., Pharmawati, M., & Hendriyani, E. 2020. Propagation Of Begonia Bimaensis Undaharta & Ardaka Using Tissue Culture Technique. Journal of Biological Sciences, 7(1): 112-122. https://doi.org/10.24843/metamorfo sa.2020.v07.i01.p15

Wattimena, G.A. 2001. Zat Pengatur Tumbuh Tanaman. Bogor: Pusat Antar Universitas Institut Pertanian Bogor.

Downloads

Published

2024-07-29

How to Cite

Welsiliana, W., & Mata, M. H. (2024). Pengaruh Hormon 2.4-D (2,4-Dichlorophenoxyacetic Acid) Terhadap Kultur Kalus Daun Tembakau (Nicotiana tabacum L). Journal Science of Biodiversity, 5(1), 39–44. https://doi.org/10.32938/jsb/vol5i1pp39-44

Issue

Vol. 5 No. 1 (2024): April 2024

Section

Articles

License

Authors who publish articles in Journal Science of Biodiversity agree to the following terms:

  1. Authors retain copyright of the article and grant the journal right of first publication with the work simultaneously licensed under a CC-BY-SA or The Creative Commons Attribution–ShareAlike License.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).