The Effect of Adding Lumut Banana Fruit Juice in Egg Yolk Citrate Diluent on The Quality of Duroc Boar Spermatozoa

Abstract

The aim of this study was to evaluate the use of Ambon green banana extract (SBPAL) in citrate-egg yolk extender (SKT) to preserve Duroc boar semen at a storage temperature of 18-20°C. The study material consisted of healthy Duroc boar semen aged 2 years, accustomed to semen collection. The experiment used a completely randomized design (CRD) with six treatments and five replications: P0 = 100% SKT, P1 = 100% SKT + 10% SBPAL, P2 = 100% SKT + 15% SBPAL, P3 = 100% SKT + 20% SBPAL, P4 = 100% SKT + 25% SBPAL, P5 = 100% SKT + 30% SBPAL. The variables measured included motility, viability, abnormalities, and spermatozoa longevity (LTH). The results showed that the P4 treatment produced the highest spermatozoa quality statistically (P<0.05) compared to the other treatments, with motility: 46.00±2.12%, viability: 53.74±2.42%, abnormality: 4.47±0.84%, and spermatozoa LTH: 55.28±2.40 hours. In conclusion, the addition of 25% SBPAL in the SKT extender effectively maintained the quality of Duroc boar spermatozoa for up to 48 hours of storage.