Quality of Landrace Semen in Yolk Citrate Cement which Plus Glucose with Different Concentrations
DOI:
https://doi.org/10.32938/jtast.v4i1.1268Keywords:
Abnormality and pH of Semen, Landrace Pig, Glucose and citrate yolk, Motility and ViabilityAbstract
The purpose of this study was to determine the effect of using yolk citrate diluent with different concentrations of glucose added on the motility, viability, abnormalities and pH of landrace pig cement.The cement used was fresh cement from a 3 year old Landrace pig stud.This study used an experimental method using a completely randomized design (CRD) with four treatments (P1 5%, P2 10%, P3 15% and P4 20%) and four replications so that there were 16 experimental units.The results showed that the concentration of glucose in the yolk citrate diluent was not significantly different (P>0.05) on the motility of spermatozoa with the average value of each treatment being P1 (65%), P2 (60%), P3 (63, 8%) and P4 (67.5%).The percentage of spermatozoa viability was very significantly different (P<0.01) between treatments, where in treatment P2 was relatively the same as treatment P3 and P4 but higher than treatment P1 with the value of each treatment P1 (83%), P2 (90.1 %), P3 (93.5%) and P4 (91.5%). Percentage of spermatozoa abnormalities resulting from analysis of variance (ANOVA) from each treatment there was a non-significant difference (P>0.05) where the values were P1 (8.2%), P2 (9.2%), P3 (8.1%) and P4 (9.2%). The pH was significantly different (P<0.05) between treatments, where P1 treatment was relatively the same as P2 but higher than P4 treatment. On the other hand, treatment P3 was relatively the same as treatment P1, P2 and P4, with the values of each treatment being P1 (8.36), P2 (7.99), P3 (7.676) and P4 (6.83). It can be concluded that the use of different glucose concentrations in the egg yolk citrate diluent with good quality, namely in the P3 treatment with a glucose concentration of 15%, the average percentage of individual motility was 64%, the percentage of live spermatozoa was 93.5%, the percentage of abnormal spermatozoa was 8.1%. and the average pH of cement is 7.68. It was concluded that the quality semen of Landrace pig could be improved optimally using glucose concentration in egg yolk citrate diluent by 15%.
Downloads
References
Affandhy, L., U. Umiyasih, dan K. Maksu`m. 1998. Evaluasi kualitas semen beku sapi Madura dengan berbagai ddiluter dan kandungan kuning telur yang berbeda. Hlm: 233-239. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner Ciawi-Bogor.
Arifiantini, R.I., dan T.L.Yusuf. 2004.Keberhasilan penggunaan tigapengencer dalam dua jenis kemasan pada proses pembekuan semen sapi Frisien Holstein.Fakultas Kedokteran hewan, Institut Pertanian Bogor, Bogor.
Arifiantini, I., T.L. Yusuf, dan D. Yanti. 2005. Kaji banding kualitas semen beku sapi Friesian Holstein menggunakan pengencer dari berbagai Balai Inseminasi Buatan di Indonesia. Animal Production 7(3):168-176.
BIB Ungaran. 2011. Instruksi kerja. Balai Inseminasi Buatan Ungaran. Jawa Tengah.
Blakely, J. dan D. H. Bade, 1992. Ilmu Peternakan. Edisi. 4. Srigandono B, Penerjemah: Gadjah Mada University Press. Yogiakarta. Indonesia.
Butar, E. 2009. Efektifitas Frekuensi Exercise Terhadap Peningkatan Kualitas Semen Sapi Simmental. http://repository.usu.ac.id/bitstream/1/09E008 98. pdf. Diakses pada 5 November 2013. Direktorat Jenderal Peternakan. 2000. SNI 01.4869.1- 2005 Semen Beku Sapi.
CasasI and AlthousGC. 2013.Theprotectiveeffect ofa17ºCholdingtimeonboarsperm plasmamembranefluidityafterexposure5ºC. Cryobiology66:69-75.
Eriksson, B.M., Petersson, H. and Rodriguez-Martinez, H. 2002. Field Fertility with Exported Boar Semen Frozen in the New Flatpack Container. Theriogenology. 58: 1065-1079.
Evans, G., dan W.M.C. Maxwell, 1987. Salamon’s Artificial Insemination of Sheeps and Goat’s. Butterworth. London
Feradis. 2010. Bioteknologi Reproduksi padaTernak. Alfabeta, Bandung.
Gadea J, 2003. Semen extenders used in the artificial insemination of swine. Spanish Journal of Agricultural Research 1 (2): 17-27.
Garner D.L, Hafez E S E. 2000. Spermatozoa and Seminal Plasma.In: Hafez E.S.E. and B. Hafez (Eds.). Reproduction in Farm Animals.7th Ed.US: Lippincott Williams & Wilkins. Pp 96-109.
Gilmore, J.A., Junying, D., Jun, T., Peter, A.T. and Crister, J.K. 1996. Osmotic Properties of Boar Spermatozoa and their Relevance to Cryopreservation. J. Reprod. Fertil. 107:87-95
Hardjosubroto. W, 1994. Aplikasi pemuliabiakan ternak dilapangan. Penerbit PT Grasindo. Jakarata.
Hafez, E.S.E. 2000. Semen Evaluation, In: Hafez, B., and E.S.E. Hafez (Eds). Reproductionin Farm Animals. 7th. Ed. Lippincot Williams and Wilkins. Philadelphia
Hafez, B. and Hafez. E. S. E. 2000. Reproduction in Farm Animals. 7thed. Lippincott Williams and Wilkins, Philadelphia.
Johnson LA, Weitze KF, Fiser P, Maxwell WMC. 2000. Storageof boar semen. Journal Animals Reproduction Science 62: 143-172.
Kvist, U., Bjo¨rndahl, L. (2002). Editorial. In: Kvist U, Bjo¨rndahl L, eds. Manualon Basic Semen Analysis. ESHRE Monographs. Oxford, United Kingdom. Oxford University Press.
Moussa M., Marinet, V., Trimeche, A. 2002. Low Density Lipoproteins Extracted from hen Egg Yolk by an Easy Method: Cryoprotective Effect on Frozen-thawed Bull Semen. Theriogenology, v.57, p.1695-1706, 2002.
Mumu, M. I. 2009. Viabilitas Semen Sapi Simmental yang Dibekukan Menggunakan Krioprotektal Gliserol. Skripsi. UniversitasTadulako. Palu
Partudihardjo. 1992. llmu reproduksi hewan. MutiaraWidya.Cetakanke-S.Jakarta
Paulenz H, Kommisrud E, and Hofmo PO. 2000. Effect of long-term storage eat different temperatures on the quality of liquid boar semen. Reprod. Dom. Anim. 35:58–85.
Panglowpahan. 2003. Influence of glucose and fruktose in the extender during long term storage of chilled canine semen. Theriogenology 62: 1498 -- 1517.
Raharjo, D. 2002. Daya Tahan Spermatozoa Semen Cair Sapi FH dalam Kemasan Straw Mini Menggunakan Pengencer Citrat Kuning Telur dan Skim Kuning Telur dengan Penambahan Fruktosa. Skripsi Fakultas Kedokteran Hewan Institut Pertanian Bogor. Bogor.
Rizal, M. 2006. Peranan beberapa jenis gula dalam meningkatkan kualitas semen beku domba Garut. Jurnal Ilmu Ternak dan Veteriner 11:123--130.
Rienprayoon C, Klangnak C, Onton S, Tretipskul C, and Tummaruk P, 2012. Acomparitive study on the efficacy of four commercial semen extenders used during the holding time on the qualities of frozen-thawed boar semen. Thai J. Vet. Med.42,195-200.
Salisbury, G.W. and N.L. Vandemark.,1985. Fisiologi Reproduksi dan Inseminasi Buatan Pada Sapi. Terjemahan. Judul Asli: (Physiologyof Reproduction and Artificial Insemination of Cattle), Penerjemah: Djanuar, R, (Ed),Gadjah Mada University Press,Yogyakarta.
Salmah, Nur., 2014. Motilitas, Persentase Spermatozoa Hidup dan Abnormalitas Spermatozoa Semen Beku Sapi Bali Pada Pengencer Andromed dan Tris Kuning Telur. Skripsi. Universitas Hassanudin. Makassar.
Setyono, 2007. Pengaruh penambahan streptomycin dalam skim kuning tekur sebagai pengencer terhadap kualitas semen ikan mas (Cyprinus CarpioL).
Sihombing, D.T.H. 1997. Ilmu Ternak Babi. Fakultas Peternakan IPB, Bogor.
Siswanto. 2006. Kualitas Semen di dalam pengencer Tris dan Natrium Sitrat dengan berbagai sumber karbohidrat dan level gliserol pada proses kriopreservasi semen Rusa Timor (Cervus timorensis). Sekolah Pasca Sarjana Institut Pertanian Bogor, Bogor. (Tesis)
Solihati, N., R. Idi., S. D. Rasad., M. Rizal. Dan M. Fitriati. 2005. Kualitas Spermatozoa Caudan Epididymis Sapi Peranakan Ongol (PO) Dalam Pengencer Susu, Tris Dan Sitrat Kuning Telur Pada Penyimpanan 4-5 oC. Jurnal Animal Produktion. 10 (1): 22-29.
Steel, R.G.D., Torrie, J.H., 1993. Prinsip dan Prosedur statistika (diterjemahkan dari: Principles and Procedure of Statistic, penerjemah: B. Sumantri). PT. Gramedia, Jakarta.
Steinbach, J. and R.H. Foote.1967. Osmotic Pressure and pH Effects on survival of frozen on liquid spermatozoa. Journal of Dairy Science 50 :205-213.
Toelihere, M., R. 1993. Inseminasi Buatan pada Ternak. Angkasa, Bandung.
Toelihere, M.R., 1985. Inseminasi Buatan pada Ternak Cetakan II. Angkasa, Bandung.
Triana, I. N. 2006. Pengaruh Waktu Inseminasi Terhadap Motilitas Dan Viabilitas Spermatozoa Pasca Inseminasi Pada Kambing. Berk. Penel. Hayati. 11: 147-150.
Wagner, H.G., and Thibier, M. 2000. World Statistics for Artificial Insemination In Small Ruminants and Swine. Proc 14th ICAR, Stockholm, Sweden. vol. 2, 15:3
Walson, P.F. and Martin, C.A. 1975.The Influences of same fractions of egg yolkon the survival of ram spermatozoa at5oC. Reprod Fertil Dev 69:856-857.
Woelders, H., A. Matthij and B. Engelan cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology 35: 93†10.
